I do love Trixie and even if I didn't it is not like I would ever want another kitten from that breeder anyway. We did a full month of metronidazole with no change so we are off it now.
This is what I found when searching the test they are doing
Once the PCR process begins, a specific process called amplification begins.
1.Denaturation, the DNA heats to 194 degrees F to separate the DNA into two strands.
2.Annealing, a pcr test is specific to the disease it is looking for. Therefore, each disease or parasite has a primer that binds to the DNA strands. Then the DNA cools to about half the temperature it was in Step 1. When step 2 is complete the lab has two separate strands of DNA. Then, the sequences marked by primers and ready to begin again.
3.The extension begins again. The temperature rises again. This time to 161.5 F. Starting where the primers marked DNA Polymerase (enzymes that produce copies) is added. After the completion of ‘extension,’ there are two identical copies of the original DNA.
4.The cycle starts over. Each time doubling the exact copies of the original DNA. After thirty to forty cycles more than one billion copies of the original DNA have been made.
The entire process is automated and completes very quickly.
PCR amplification is only part of the identifying test, however. Once the amplification completes, the amplified segments are compared to other nucleotide segments from a known source (for example, dna from giardia).
There is Feline PCR test available for the following diseases and parasites at labs in the United States and Europe.
•Feline Corona Virus
•Feline Infectious Peritonitis
•Feline Leukemia Virus
The benefits of a PCR test includes a lower chance of false negative results and early detection of infections. In addition, PCR tests are highly sensitive since they differentiate pathogen strains that appear similar.
Please ask your breeder for proof of PCR testing, it is important to know the health of your kitten.